Phytochemical studies, in vitro antioxidant and antiproliferative of the stem bark of Boswellia dalzielii hutch
Abstract
This work aims to evaluate the total phenolic and flavonoid contents, and antioxidant and Antiproliferative activities of the stem bark of Boswellia dalzielii. Hundred gram (100 g) of methanolic extract was re-dissolved in 70% methanol and partitioned exhaustively with different solvent hexane and ethyl acetate in a separating funnel; and this method gave three fractions, hexane fraction, ethyl acetate fraction and aqueous extract. The ethyl acetate fraction was subjected to Accelerated Gradient Chromatographic due to its higher activity over the hexane fraction and four sub-fractions were obtained. Standard methods were used to determine flavonoid and phenolic contents of the methanolic, aqueous, ethyl acetate and hexane fractions and their sub-fractions. Standard methods were used to determine flavonoid and phenolic contents of the methanolic, aqueous, ethyl acetate and hexane extracts and their sub-fractions. The antioxidant property of the extracts was determined using DPPH radical scavenging and FRAP assay. Growth inhibitory activity was carried out on the crude extracts and sub-fractions using Sorghum bicolor seeds. The phenolic content was found to be highest in sub-fraction C (481.20 ± 10.13 mg GAE/g) and flavonoid contents were found to be highest in methanolic extract (142.17 ± 4.82 mg RE/g). Boswellia dalzielii stem bark exhibited antioxidant capacity; and the highest antioxidant activities were recorded from aqueous extract with the IC50 1.58 and methanol extract IC50 1.99 using DPPH. FRAP assay exhibited antioxidant capacity with EC50 1.00 for aqueous extract and sub-fraction D EC50 1.25. The antiproliferative, sub-fractions C and D at 125 µg/ml gave the highest percentage of inhibition (90%) followed by sub-fraction B (50%) at 250 µg/ml. These results further showed that the stem bark of Boswellia dalzielii has antioxidant activities and antiproliferative activity on the seeds of Sorghum bicolor; and therefore possess likely an anticancer component which needs further anticancer screening.
Downloads
References
plants used by traditional healers in western areas of burkinafaso. World Jour. of pharm
and Pharmace. Sci. 2(6): 6706-6719.
2 Alemika, T. O. E, Onawunmi G.O. and Olugbade T. A., (2006): Antibacterial phenolics from
Boswellia dalzielii. Nig. Jour. Nat. Prod and Med. 10: 108-110.
3 Alemika, T. O. E Onawunmi G.O. and Olugbade T. A., (2004): Isolation and characterization
of incensole from Boswellia dalzielii stem bark. Jour. of Pharm. and Bioresources.
1(1):7-11.
4 Emmanuel, A. O, Adediji, J. A, Ehimigbai A. R. O, (2015): Historical effects of aqueous
extract of Boswellia dalzielii stem bark on the testes of adult wistar rats. Jour. of pharm.
and Sci. innov. 4(5):2277-4572.
5 Balogun O, (2013): Chemical studies and hypoglycemic activity of the aqueous stem bark
extract of Boswellia Dalzielii HUTCH. M.Sc. Thesis. University of Jos. Nigeria.
6 Younoussa L., Nchiwan N.E, Danga Y.S.P., Charles O. E, (2014): Larvicidal activity of
Annonasenegalensis and Boswellia dalzielii leaf fractions against Aedesaegypti (Diptera:
Culicidae). Int. Jour. of Mosq. 1(4): 25-29.
7 Aliyu R., Donatien G. and Kiri H.J., (2007): The effects of Boswellia dalzielii (Burseraceae)
aqueous bark extract on Rat liver function. Asian Jour. of Bioc. 2(5): 359-363.
8 Baoua, M., Fayn, J. and Bassere, J, (1976): Preliminary phytochemical testing for some
medicinal plants of Niger; Plante Med Phytother. 10: 251-266.
9 Dalziel, J.M, (1937): The useful plants of West Tropical Africa; Crown Agents for Oversea
Governments and Administration, London. 314-8, 345-6.
10 Burkill, H.M, (1965): Useful plants of West Tropical Africa; UK: White Friars Press. (1) 300.
11 Olukemi, M. A; Kandakai, Olukemi Y.T and Mawak, J. D (2005): Antibacterial activity of
the stem bark of Boswellia dalzielii. Jour. Pharmacy Bioresource.; 2:131-136.
12 Hamidpour R., Hamidpour S, Hamidpour M, Shahlari M& Hamidpour R. (2015). Chemistry,
Pharmacology and Medicinal Property of Frankincense (Boswellia Species): From the
Selection of Traditional Applications to the Novel Phytotherapy for the Prevention and
Treatment of Serious Diseases. Glo. Jour. of Med. 15(3):1.0
13 Evans, W.C. (1989): Trease and Evans Pharmacognosy; 13th edition, ELBS/BaillereTindall,
United Kingdom, pp. 474-475.
14 Ahmed A. Zaki, et al., (2014): Cardio-protective and antioxidant effects of oleogum resin
"Olibanum" from Boswellia carteri Birdw. (Bursearceae). Chinese Jour. of Natural Med,
12(5): 0345−0350.15 Ahmed S, Md. Alam A, Mohd. Shahabuddin, Md. Khan I, Ali H: Versatile Pharmacological
Action and Compound Formulation of Kundur in Unani Medicine (2014): A Review. Int.
Jour. Pharmacognosy; 1(10) 627-631.
16 Gulcin I, Topal F.S., Sarıkaya B.O., Bursal E., GokhanBilsel and Goren A.C, (2011):
Polyphenol Contents and Antioxidant Properties of Medlar (Mespilusgermanica L.).Rec.
Nat. Prod. 5(3) 158-175
17 Adedapo A.A, Jimoh F.O, Afolayan A.J, Masika P.J, (2008): Antioxidant activities and
phenolic contents of the methanol extract of the stems of Acokantheraoppositifolia and
Adeniagummifera. BMC Complement and Altern Med. 8: 54.
18 Huma M, (2014): Antioxidant and Antimicrobial Activities of Selected Plants of Pothohar
Plateau. Ph.D. Thesis. University Of Agriculture Faisalabad, Pakistan.
19 Odumosu P, Ojerinde S, Egbuchiem M, (2015): Polyphenolic contents of some instant tea
brands and their anti-oxidant activities. Jour. App Pharm Sci. 5 (09): 100-105.
20 Kalava S. V. and Menon S. G, (2012): In vitro free radical scavenging activity of aqueous
extract from the Mycelia of Volvariella Volvacea (Bulliard EX fries) singer. Int. Jour. of
Curr Pharm Res. 4(3):94-100
21 Ikpefan E. O. and Ayinde B. A, (2013): Preliminary Phytochemical and Comparative Growth
Inhibitory Assay of the Aqueous and Chloroform Fractions of the Methanol Extract of
The Leaves of Persea Americana Mill Lauraceae. American Jour. of Phytome and
Clinical Therapeutics. 1(7):594-604
22 Patel R. M. and Patel N. J, (2011): In vitro antioxidant activity of coumarin compounds by
DPPH, Super oxide and nitric oxide free radical scavenging methods. Jour. of Advanced
Pharm. Educ. & Research. 1:52-68.
23 Borkataky M & Sood K, (2014): Antibacterial, antioxidant and cytotoxic activities of
cinnamomum tamala nees. Leaves. Inter. Journ. of Medicine and Pharmaceutical
Sciences (IJMPS), 4(6):55-62.
24 Ojerinde S.J. (2010). Chemical and biological studies of the gum-resin of Boswellia Daziellii
hutch; M.Sc. Thesis. University of Jos. Nigeria
25 Dildar A., Muhammad M.K. and Ramsha S, (2015): Comparative Analysis of Phenolics,
Flavonoids, and Antioxidant and Antibacterial Potential of Methanolic, Hexanic and
Aqueous Extracts from Adiantum caudatum Leaves. Antioxidants. 4, 394-409
26 Basar, S, (2005): Phytochemical investigations on Boswellia species: Comparative studies on
the essential oils, pyrolysates and Bosswellic acids of B. carterii Birdw., B. serrata
Roxb., B. frereana Birdw., B. neglecta S. Moore and B. rivae Engl. Ph. D Dissetation,
University of Humburg, Germany
Author(s) and co-author(s) jointly and severally represent and warrant that the Article is original with the author(s) and does not infringe any copyright or violate any other right of any third parties, and that the Article has not been published elsewhere. Author(s) agree to the terms that the GPH Journal will have the full right to remove the published article on any misconduct found in the published article.
