ISOLATION AND IDENTIFICATION OF ANTHRACHNOSE PATHOGEN ON APPLES (Malus domestica BORKH)
Abstract
Anthracnose disease in post-harvest apples is very disturbing to consumers, especially the people on the island of Bali, where most of the time during religious ceremonies always use fruit as an offering material. Anthracnose is a disease that damages fruit with symptoms of brown to black spots on the skin of the fruit which can reduce the quality of the fruit. PCR analysis results found 650 bp sized fragments which were successfully amplified from 6 mushroom samples using ITS/ITS4 universal primers. The amplified DNA sample is then used for the sequencing stage, to determine the fungal species. The results of the sequence analysis confirmed that the identity of the fungus in the sample was Colletotrichum gloeosporioides with 78-100% homology respectively to several C. gloeosporioides isolates and out group in the genebank.
Downloads
References
Bajpai VK, SM. Al-Reza, UK. Choi, JH. Lee, and SC. Kang. 2009. Chemical composition, antibacterial and antioxidant activities of leaf essential oil and extracts of MetasequioaglyptostroboidesMiki ex Hu. Food Chem Toxicol. 47: 1876-83.
Dowling M., N. Peres, S. Villani and G. Schnabel. 2020. Managing Colletotrichum on Fruit Crops: A Compelx Challenge. Plant Disease 104: 2301-2316.
Doyle, J.J. and J.L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19: 11-15.
Khodadadi F., J.B.González, P.L. Martin, E. Giroux,G.J. Bilodeau, K.A. Peter, V.P. Doyle and S.G.Aćimović. 2020. Scientific Reports 10:11043.
Leonberger K., M. McCulloch, and N. W Gauthier. 2019. Bitter Rot of Apple. Plant Pathology Fact Sheet. University of Kentucky, College of Agriculture, Food and Environment. Extension Plant Pathology.
Onofre, S.B. and D. Antoniazzi. 2014. Behavior of the Fungus Colletotrichum gloeosporioides (Penz&Sacc.), Which CausesBitter Rot in Apples after Harvesting. Advances in Microbiology 4: 202-206.
Park M.S., B.R. Kim, I.H. Park and S.S. Hahm. 2018. First Report of Two Colletotrichum Species Associated with Bitter Rot on Apple Fruit in Korea – C. fructicola and C. siamense. Mycobiology 46(2): 154-158.
Sharma M. and S. Kulshrestha. 2015. Colletotrichum gloeosporioides: an Anthracnose Causing Pathogen of Fruits and Vegetables. DOI:10.13005/bbra/1776.
Zhang R., S.F. Wang, J.Q. Cui and G.Y.Sun. 2008. First Report of Bitter Rot Caused by Colletotrichum acutatum on Apple in China. Disease Notes 92(10): 1474.
Copyright (c) 2023 I Made Sudarma, Ni NengahDramiati & dan I Made Agus Dhramadiatmika
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Author(s) and co-author(s) jointly and severally represent and warrant that the Article is original with the author(s) and does not infringe any copyright or violate any other right of any third parties, and that the Article has not been published elsewhere. Author(s) agree to the terms that the GPH Journal will have the full right to remove the published article on any misconduct found in the published article.
