Natural Bioactive Compounds as Metabolic Modulators in Cultured Fish: A Critical Review of Lipid Homeostasis, Oxidative Stress, and Cellular Responses
Abstract
High-fat diets (HFDs) are increasingly used in aquaculture to increase dietary energy density and spare protein for growth, but excessive lipid supply can promote hepatic lipid accumulation, oxidative stress, inflammation, intestinal dysfunction, and impaired metabolic homeostasis. Natural bioactive compounds have therefore attracted attention as functional feed additives capable of improving metabolic resilience. This critical review evaluates evidence from fish studies on curcumin, resveratrol, chlorogenic acid, berberine, quercetin, tea polyphenols, hydroxytyrosol, glycyrrhetinic acid, and related plant-derived compounds used under high-fat or high-lipid feeding conditions. Particular attention is given to mechanisms involving PPAR-mediated fatty-acid oxidation, SREBP/FAS-regulated lipogenesis, AMPK-associated energy sensing and mitochondrial quality control, Nrf2-linked antioxidant defense, and NF-κB-associated inflammatory signaling. Across the available evidence, several natural bioactives reduce hepatic or circulating lipid accumulation and improve antioxidant, inflammatory, histological, or metabolic indicators, although growth responses are less consistent. Responses depend strongly on fish species, dietary lipid level and source, bioactive dose, feeding duration, compound purity, and the physiological endpoint examined. Current evidence therefore supports selected natural bioactives primarily as metabolic modulators rather than universal growth promoters. Future research should emphasize standardized HFD models, dose-response designs, chemical characterization of extracts, correct identification of the experimental unit, mechanistic validation, and testing under commercial farming conditions.
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References
Desouky, H. E., Jiang, G.-Z., Zhang, D.-D., Abasubong, K. P., Yuan, X., Li, X.-F., & Liu, W.-B. (2020). Influences of glycyrrhetinic acid (GA) dietary supplementation on growth, feed utilization, and expression of lipid metabolism genes in channel catfish (Ictalurus punctatus) fed a high-fat diet. Fish Physiology and Biochemistry, 46(2), 653–663. https://doi.org/10.1007/s10695-019-00740-4
Dong, Y.-Z., Li, L., Espe, M., Lu, K.-L., & Rahimnejad, S. (2020). Hydroxytyrosol attenuates hepatic fat accumulation via activating mitochondrial biogenesis and autophagy through the AMPK pathway. Journal of Agricultural and Food Chemistry, 68(35), 9377–9386. https://doi.org/10.1021/acs.jafc.0c03310
Dong, Y.-Z., Xia, T., Lin, J.-B., Wang, L., Song, K., & Zhang, C.-X. (2021). Quercetin attenuates high-fat diet-induced excessive fat deposition of spotted seabass (Lateolabrax maculatus) through the regulatory for mitochondria and endoplasmic reticulum. Frontiers in Marine Science, 8, 746811. https://doi.org/10.3389/fmars.2021.746811
Hu, J., Xiong, L.-F., Zuo, Y.-F., Xing, L., & Lin, Y.-T. (2024). Effects of dietary berberine on growth performance, antioxidant capacity, lipid metabolism and intestinal health in zebrafish fed high-fat diet. Acta Agriculturae Jiangxi, 36(10), 54–62. https://doi.org/10.19386/j.cnki.jxnyxb.2024.10.008
Ji, R., Li, Y., Li, X., Xiang, X., Zhang, L., Zhu, S., Yang, B., Chen, Y., Zhang, Y., & Ai, Q. (2018). Effects of dietary tea polyphenols on growth, biochemical and antioxidant responses, fatty acid composition and expression of lipid metabolism related genes of large yellow croaker (Larimichthys crocea). Aquaculture Research, 49, 1210–1218. https://doi.org/10.1111/are.13574
Ji, R., Xu, X., Li, X., et al. (2021). Effects of dietary curcumin on growth, antioxidant capacity, fatty acid composition and expression of lipid metabolism-related genes of large yellow croaker fed a high-fat diet. British Journal of Nutrition, 126(3), 345–354. https://doi.org/10.1017/S0007114520004171
Jia, R., Hou, Y., Zhang, L., Li, B., & Zhu, J. (2024). Effects of berberine on lipid metabolism, antioxidant status, and immune response in liver of tilapia (Oreochromis niloticus) under a high-fat diet feeding. Antioxidants, 13(5), 548. https://doi.org/10.3390/antiox13050548
Li, Q., Zheng, Y., Sun, Y., & Xu, G. (2023). Resveratrol attenuated fatty acid synthesis through MAPK-PPAR pathway in red tilapia. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 268, 109598. https://doi.org/10.1016/j.cbpc.2023.109598
Ma, J., Kong, L., Zhou, S., Lin, H., Lin, Y., Qin, H., Long, Z., Liu, L., Huang, Z., & Li, Z. (2023). Effect of supplementation of chlorogenic acid to high-fat diet on growth, lipid metabolism, intestinal and hepatic histology, and gut microbiota of spotted sea bass (Lateolabrax maculatus). Metabolites, 13(10), 1067. https://doi.org/10.3390/metabo13101067
Naiel, M. A. E., Negm, S. S., Ghazanfar, S., Shukry, M., & Abdelnour, S. A. (2023). The risk assessment of high-fat diet in farmed fish and its mitigation approaches: A review. Journal of Animal Physiology and Animal Nutrition, 107(3), 948–969. https://doi.org/10.1111/jpn.13759
Wang, X., Huang, Z., Kong, L., et al. (2026). Effects of curcumin supplementation in high-fat diet on growth performance, antioxidant capacity and lipid metabolism of spotted sea bass (Lateolabrax maculatus). Fish Physiology and Biochemistry, 52(3). https://doi.org/10.1007/s10695-026-01689-x
Wu, D., Li, J., Fan, Z., Wang, L., & Zheng, X. (2022). Resveratrol ameliorates oxidative stress, inflammatory response and lipid metabolism in common carp (Cyprinus carpio) fed with high-fat diet. Frontiers in Immunology, 13, 965954. https://doi.org/10.3389/fimmu.2022.965954
Zheng, Y., Shi, Y., Yang, X., Gao, J., Nie, Z., & Xu, G. (2022). Effects of resveratrol on lipid metabolism in liver of red tilapia Oreochromis niloticus. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 261, 109408. https://doi.org/10.1016/j.cbpc.2022.109408
Zheng, Y., Shao, N., Yang, X., Shi, Y., & Xu, G. (2024). Resveratrol ameliorates intestinal lipid metabolism through the PPAR signaling pathway in high-fat diet-fed red tilapia (Oreochromis niloticus). Fish & Shellfish Immunology, 145, 109302. https://doi.org/10.1016/j.fsi.2023.109302
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