FIRST REPORT: MOLECULAR IDENTIFICATION OF THE CAUSES OF ANTHRACNOSE DISEASE IN RAMBUTAN FRUITS IN BALI
Abstract
Anthracnose disease on post-harvest rambutan fruit is often found in traditional markets as well as freshly picked and post-harvest fruit. The disease really interferes with the appearance of the fruit, the skin color of the fruit is black and is not good for use as offerings or consumption. Molecular search results found that a 650 bp DNA fragment was successfully amplified from 5 fungal samples using universal primers ITS1/ITS4. The DNA samples resulting from the amplification are then used for the sequencing stage to determine the fungal species. Sequencing analysis confirmed that the identity of the fungus in the sample was Colletotrichum fructicola with 68-100% homology to several C. fructicola isolates and out groups in the genebank. Further phylogenetic analysis showed that C. fructicola isolates formed three groups. The C. fructicola_Bali isolate formed a group with isolates from Taiwan. The second group consists of isolates from China, while the third group consists of isolates from Japan. Meanwhile, outside the cluster there are 3 species of Colletotrichum including C. siamense, C. musae, and C. gloeosporioides. As an outgroup isolate, the Lasiodiplodiatheobromae isolate originating from Indonesia was used.
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References
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