TRICHODERMA SP. AS ENDOPHYTIC FUNGI ONE CANDIDATE FOR POD ROT DISEASE COTROL OF COCOA
Abstract
Black pod rot of cocoa is one of the most dangerous diseases causing crop losses in cocoa plants around the world. The disease is caused by the fungus Phytophthora palmivora (Butler) Butler, the disease needs to be controlled through biocontrol with the Trichoderma sp. Is an endophytic fungus found in cocoa plant stems. The results showed that the isolate of endophytic fungi found both from the stem, leaf and skin of healthy cocoa fruit was 15 isolates, from 15 isolates having in vitro inhibitory ability were collected five types of isolates such as stem endophytic fungi 3 (Trichoderma sp.) of 95.92 ± 3.90%, followed by leaf endophytic 3 (Oidium sp.) of 93.33%, stem endophytic 5 (Botryoderma sp.) of 91.11%, stem endophytes 1 (Micelia strerilia) equal to 89,62 ± 1,29%, and last end of skin of fruit 1 (Septocylindrium sp.) equal to 73,33%. Test results in vivoTrichoderma sp. best in pressing with the lowest attack percentage of 36.25 ± 2.89%, and the field test showed that Trichoderma sp. with all dilution treatments having a very significant effect on control with disease incidence that can be saved of 83.33%.
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References
[2]. Vanegtern, B.; M. Rogers & S. Nelson. 2015. Black Pod Rot of Cacao Caused by Phytophthora palmivora. Plant Disease 108: 1−5.
[3] Phillips-Mora, W. and R. Cerda. 2009. Catalog Cacao Diseases in Cetral America. Technical Sries. Technical Manual No.93. Tropoical Agricultural Research and Higher Edication Center (CATIE) Turrialba, Costa Rica.
[4]. Jackson, G.HV.H. and J.G., Wright. 2001. Black pod and cancer of cocoa. Plant Protectuon Service. Secretariat of the Pasific Community. Pest Advisory Leaflet No. 7.
[5]. Rubini M. R, R. T. Silva-Ribeiro, A.W.V. Pomella, C.S. Maki, W.L. Araújo, D. R. dos Santos and J.L. Azevedo. 2005. Diversity of endophytic fungal community of cacao (Theobroma cacao L.) and biological control of Crinipellis perniciosa, causal agent of Witches' Broom Disease. Int. J. Biol. Sc. 1:24-33.
[6]. Hanada, R.E., Pomella, A.W.V., Costa, H.S., Bezerra, J.L.,Loguercio, L.L., & Pereira, J.O. (2010). Endophyticfungal diversity in Theobroma cacao (cacao) and T. grandiflorum (cupuacu) trees and their potential for growth promotion and biocontrol of black-pod disease. Fungal Biology, 114, 901–910.
[7]. Bailey B.A., M. D. Strem, and D. F. Wood. 2009. Trichoderma species form endophytic associations within Theobroma cacao trichomes. Mycological Research 113(Pt 12): 1365-76.
[8]. Samson, R.A., E.S. Hoekstra, and C. A.N. Van Oorschot. 1981. Introduction to Food- Borne Fungi. Centraalbureau Voor-Schimmelcultures. Institute of The Royal Netherlands. Academic of Arts and Sciences.
[9]. Pitt, J.I. and A.D. Hocking. 1997. Fungi and Food Spoilage. Blackie Avademic and Professional. Second Edition. London-Weinhein-New York-Tokyo-Melboune- Madras
[10]. Barnett, H.L. and B.B. Hunter. 1998. Illustrated Genera of Imperfect Fungi. APS Press.
[11]. Indrawati. G., R.A. Samson, K. Van den Tweel-Vermeulen, A. Oetari dan I. Santoso. 1999. Pengenalan Kapang Tropik Umum. Yayasan Obor Indonesia. Universitas Indonesia (University of Indonsia Culture Collection) Depok, Indonsia dan Centraalbureau voor Schirmmelcultures, Baarn, The Netherlands (Indonesian language).
[12]. Dolar, F.S. 2001. Antagonistic effect of Aspergillus melleus Yukawa on soilborne pathogens of Chickpea. Tarim Bilimleri Dergisi, 8(2): 167-170.
[13]. Mojica-Marin, V., H. A. Luna-Olvera, C. Fco, Sandoval-Coronado, B.Pereyra- Alférez, H. Lilia, Morales-Ramos, E. Carlos, Hernández-Luna and G. O. Alvarado-Gomez. 2008. Antagonistic activity of selected strains of Bacillus thuringiensis against Rhizoctonia solani of chili pepper. African Journal of Biotechnology, 7 (9): 1271-1276.
[14]. Chen H., Chen T., Giudici P., Chen F. (2016). Vinegar functions on health: constituents, sources, and formation mechanisms. Compr Rev. Food Sci. F 15, 1124–1138.
[15]. Shentu X, Zhan X, Ma Z, Yu X, & Zhang C. 2014. Antifungal activity of metabolites of endophytic fungus Trichoderma brevicompactum from garlic. Braz. J. Microbiol. 45(1): 248-254.
[16]. Hermosa R., A. Viterbo, I. Chet, and E. Monte. 2012. Plant-beneficial effects of Trichoderma and of its genes. Microbiology 158(Pt 1): 17-25.
[17]. Waghunde R., R.M. Shelake, and A.N. Sabalpara. 2016. Trichoderma: A significant fungus for agriculture and environment. African Journal of Agricultural Research 11(22): 1952-196.
[18]. Saha, A.K., A. Ray, and P. Das. 2013. Fungal colonization of philloplane of Psidium guineese Sw. growing in Suryamanigar, Tripura Northeast India. International Journal of Basic and Applied Chemical Sciences 3(1): 62-67.
[19]. Resende, M. L. V., Nojosa, G. B. A., Cavalcanti, L. S., Aguilar, M. A. G., Silva, L. H. C. P., Perez, J. O., et al. 2002. Induction of resistance in cocoa against Crinipellis perniciosa and Verticillium dahliae by acibenzolar-S-methyl (ASM). Plant Pathol. 51, 621–628
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