Effects of Smoothies on Semen Parameters, Nitric Oxide and Total Protein in Male Wistar Rats

  • Gbaranor K. B. Department of Human Physiology, College of Medical Sciences, Rivers State University, Port Harcourt, Nigeria
  • Adomale L. F. Department of Human Physiology, College of Medical Sciences, Rivers State University, Port Harcourt, Nigeria
  • Tamuno-Opubo A. Department of Human Physiology, College of Medical Sciences, Rivers State University, Port Harcourt, Nigeria
  • Dimkpa B. M. Department of Community Medicine, University of Port Harcourt Teaching Hospital, Rivers State, South-South, Nigeria
  • Emmanuel D. F. Department of Human Physiology, College of Medical Sciences, Rivers State University, Port Harcourt, Nigeria
  • Baridam G. D. Department of Obstetrics and Gynaecology, University of Port Harcourt Teaching Hospital, Rivers State, South-South, Nigeria
  • Chris-Biriwo H. Department of Human Physiology, College of Medical Sciences, Rivers State University, Port Harcourt, Nigeria
  • Dimkpa C. Department of Paediatrics & Child Health, College of Medical Sciences, Rivers State University, Rivers State, South, Nigeria
  • Amuduaghan A. E., Department of Family Medicine, University of Port Harcourt Teaching Hospital, Rivers State, South-South, Nigeria
  • Oyadotun M., Department of Community Medicine, University of Port Harcourt Teaching Hospital, Rivers State, South-South, Nigeria
  • Tobin B. Department of Community Medicine, University of Port Harcourt Teaching Hospital, Rivers State, South-South, Nigeria
  • Anika  Ifeoma Eunice Department of Community Medicine, University of Port Harcourt Teaching Hospital, Rivers State, South-South, Nigeria
  • Nelson I. E Department of Human Physiology, College of Medical Sciences, Rivers State University, Port Harcourt, Nigeria
Keywords: Effects, Smoothies, Semen, Parameters, Nitric Oxide

Abstract

Fruits are very important to humans and they have several essential nutrients that are important to improve their overall health. The aim of this study is to investigate the effects of smoothies (banana, apple and pineapple) on semen parameters and nitric oxide in male Wistar rats. Thirty (30) animals weighing 130kg to 180kg were randomly selected into 6 groups with 5 animals per group. Group 1 received 5mls of distilled water, group 2 received 1ml/kg (low dose) of smoothies, group 3 received 2ml/kg (medium dose), group 4 received 3ml/kg (high dose), group 5 received 400mg/kg of monosodium glutamate, group 6 received 400mg/kg of monosodium-glutamate and 3ml/kg (high dose) of smoothies co-administered. Administration was carried out for 14 days and on the 15th day, the animals were sacrificed, semen was harvested and 5ml blood was collected via cardiac puncture. Statistical analysis was done using ANOVA and expressed as Mean±SEM. Statistically P < 0.05 was said to be significant. SPSS version 26 was used. Results from the study showed significant increase in sperm morphology and sperm volume and decrease in sperm count and sperm viability. There was significant increase in the level of nitric oxide dose-dependent and thus could aid erection in male.

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References

Agarwal, A., Mulgund, A., Hamada, A., & Chyatte, M. (2015). A unique view on male infertility around the globe. Reproductive Biology and Endocrinology, 13(1). https://doi.org/10.1186/s12958-015-0032-1
Babakhanzadeh, E., Nazari, M., Ghasemifar, S., & Khodadadian, A. (2020). Some of the Factors Involved in Male Infertility: A Prospective Review. International journal of general medicine, 13, 29–41. https://doi.org/10.2147/IJGM.S241099
Banihani, S. A., & Aljabali, S. M. (2020). Seminal plasma vitamin B 6 levels in men with asthenozoospermia and men with normal sperm motility, a measurement using liquid chromatography with tandem mass spectrometry. Andrologia, 52(4). https://doi.org/10.1111/and.13556
Burnett AL (2007). The Journal of Clinical Hypertension. The Role of Nitric Oxide in Erectile Dysfunction: Implications for Medical Therapy. Published online doi: 10.1111/j.1524-6175.2006.06026.x
Durairajanayagam, D. (2018). Lifestyle causes of male infertility. Arab Journal of Urology, 16(1), 10–20. https://doi.org/10.1016/j.aju.2017.12.004
Eisenberg, M. L., Kim, S., Chen, Z., Sundaram, R., Schisterman, E. F., & Louis, G. M. B. (2013). The relationship between male BMI and waist circumference on semen quality: data from the LIFE study. Human Reproduction, 29(2), 193–200. https://doi.org/10.1093/humrep/det428
Gundersen, T. W., Jørgensen, N., Andersson, A., Bang, A. K., Nordkap, L., Skakkebæk, N. E., Priskorn, L., Juul, A., & Jensen, T. K. (2015). Association between use of marijuana and male reproductive hormones and semen quality: a study among 1,215 healthy young men. American Journal of Epidemiology, 182(6), 473–481. https://doi.org/10.1093/aje/kwv135
Igwebuike U, Ochiogu I, Ihedinihu B, Ikokide. J, and Idika. I. (2011). The effects of oral administration of monosodium glutamate (msg) on the testicular morphology and cauda epididymal sperm reserves of young and adult male rats. Vet. Arh. 81, 525–534.
Kelvin P Davies (2015). Future Sci. OA (2015) 1(1), FSO53
Ma, Y., He, X., Qi, K., Wang, T., Qi, Y., Cui, L., Wang, F., & Song, M. (2019). Effects of environmental contaminants on fertility and reproductive health. Journal of Environmental Sciences, 77, 210–217. https://doi.org/10.1016/j.jes.2018.07.015
Nargund, V. (2015). Effects of psychological stress on male fertility. Nature Reviews Urology, 12(7), 373–382. https://doi.org/10.1038/nrurol.2015.112
Nayanatara A, Vinodini N, Damodar G, Ahemed B, Ramaswamy C, Shabarianth and Ramesh B (2008). Role of ascorbic acid in monosodium glutamate mediated effect on testicular weight, sperm morphology and sperm count, in rat testis. Journal of Chinese Clinical Medicine. (3) 1.
Onakewhor J, Oforofuo I, and Singh S (1998). Chronic administration of Monosodium glutamate induces Oligozoospermia and glycogen administration in Wistar rat testes. Africa Journal of Reproduction and Health 2(2): 190-197.
Ou, M. (2009). Acrosome reaction in the headattached human sperm. Andrologia, 26(1), 17-20. https://doi.org/10.1111/j.1439- 0272.1994.tb00747.x
Qi, X., Wang, K., Zhou, G., Xu, Z., Yu, J., & Zhang, W. (2016). The role of testicular artery in laparoscopic varicocelectomy: a systematic review and meta-analysis. International Urology and Nephrology, 48(6), 955–965. https://doi.org/10.1007/s11255-016-1254-7
Ricci, E., Beitawi, S. A., Cipriani, S., Candiani, M., Chiaffarino, F., Viganò, P., Noli, S., & Parazzini, F. (2017). Semen quality and alcohol intake: a systematic review and meta-analysis. Reproductive Biomedicine Online, 34(1), 38–47. https://doi.org/10.1016/j.rbmo.2016.09.012
Salonia, A., Bettocchi, C., Boeri, L., Capogrosso, P., Carvalho, J., Çilesiz, N. C., Cocci, A., Corona, G., Dimitropoulos, K., Gül, M., Hatzichristodoulou, G., Jones, T. H., Kadıoğlu, A., Martínez-Salamanca, J. I., Milenković, U., Modgil, V., Russo, G. I., Şerefoğlu, E. C., Tharakan, T., . . . Minhas, S. (2021). European Association of Urology Guidelines on Sexual and Reproductive Health—2021 Update: Male Sexual Dysfunction. European Urology, 80(3), 333–357. https://doi.org/10.1016/j.eururo.2021.06.007
Santiago B, Claudio C and Susana K. (2000). Definition and causes of infertility
Sharma, R., Harlev, A., Agarwal, A., & Esteves, S. C. (2016). Cigarette Smoking and semen quality: A new meta-analysis examining the effect of the 2010 World Health Organization laboratory methods for the examination of human semen. European Urology, 70(4), 635–645. https://doi.org/10.1016/j.eururo.2016.04.010
Slavin, J. L., & Lloyd, B. (2012). Health benefits of fruits and vegetables. Advances in Nutrition, 3(4), 506–516. https://doi.org/10.3945/an.112.002154
Sorensen, M. Zinc, (1999). Magnesium and calcium in human seminal fluid: relations to other semen parameters and fertility. Molecular Human Reproduction, 5(4), 331-337. https://doi.org/10.1093/molehr/5.4.331
Sunday, A.G., O.E. Ifeanyi and O.F. Uzor, (2014). Wound healing potential of water and ethanol extract of fresh leaves and bark Ofgambia albidum in albino rats. IOSR J. Dental Med. Sci., 13: 16-22
Taha, E. A., Ez-Aldin, A. M., Sayed, S. K., Ghandour, N., & Mostafa, T. (2012). Effect of smoking on sperm vitality, DNA integrity, seminal oxidative stress, zinc in fertile men. Urology, 80(4), 822–825. https://doi.org/10.1016/j.urology.2012.07.002
World Health Organization. (2023). Infertility. World Health Organization. https://www.who.int/health-topics/infertility#tab=tab_1
Yakubu MT, Akanji MA, Olajidi AT (2005). Aphrodisiac potentials of the aqueous extract of Fadogia agrestis (Schweinf. Ex Heirn) stem in male albino rats. Asian Journal Andrology 7(4):399-404.
Published
2024-04-24
How to Cite
K. B., G., L. F., A., A., T.-O., B. M., D., D. F., E., G. D., B., H., C.-B., C., D., A. E., A., M., O., B., T., Ifeoma Eunice, A., & I. E, N. (2024). Effects of Smoothies on Semen Parameters, Nitric Oxide and Total Protein in Male Wistar Rats. GPH-International Journal of Biological & Medicine Science, 7(03), 54-62. https://doi.org/10.5281/zenodo.11099610

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