STUDY OF LETHAL DOSE (LD50) DETERMINATION AND BODY/ ORGAN WEIGHT OF RATS ADMINISTERED AQUEOUS EXTRACT OF EQUAL WEIGHT OF PAWPAW (Carica papaya) AND SOURSOP (Annona muricata)LEAVE

  • IHIMIRE INEGBENOSE GODWIN AMBROSE ALLI UNIVERSITY BIOCHEMISTRY DEPARTMENT P.M.B 14 EKPOMA, NIGERIA
  • EIGBOBO MARY UGUNNUSH AMBROSE ALLI UNIVERSITY BIOCHEMISTRY DEPARTMENT P.M.B 14 EKPOMA, NIGERIA
  • CHIEDOZIE KELVIN OJEBAH DEPARTMENTAL OF CHEMICAL SCIENCE, DELTA STATE UNIVERSITY OF SCIENCE AND TECHNOLOGY OZORO
  • RUFUS OMO FORTUNATE AMBROSE ALLI UNIVERSITY BIOCHEMISTRY DEPARTMENT P.M.B 14 EKPOMA, NIGERIA
DOI: https://doi.org/10.5281/zenodo.7923212
Keywords: Lethal dose, Toxicity, Concentrated, Body weight, Organ weight

Abstract

This study determineslethal dose toxicity (LD50)and effect on body/organ of rat administered equal weight of pawpaw (Carica papaya) and soursop (Annona muricata) leaves extra. About 125g each of the sample where mixed, extracted with 200ml of ethanol in triplicate and concentrated. The concentrated sample was used to prepare different dilution for the study. LD50 was asserted with a concentration range of 6.67-60.5mg in 150g rats. The effect on body weight and organ weight were studied with concentration range of 27.60mg/kg to 70.43 mg/kg body weight of rats. The LD50 was found to be 54.11mg/150g rats (equivalent to 3607.52mg/kg body weight). With exception of rat in group D (i.e., 10% above safe dose), rats administered the respective extract recorded non-significant (p>0.05) higher weight compared to rats in control group. The  study suggest that the extract is slightly toxic, not convient to transport but had insignificant effect on body/ organ weight.

Downloads

Download data is not yet available.

References

Adamson, B., Norman, T.M., Jost, M., Cho, M.Y., Nuñez, J.K., Chen, Y. and Weissman, J.S. (2016). A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response. Cell. 167(7): 1867-1882.
Anuar, N.S., Zohari, S.S., Taib, I.A. and Rahman, M.T. (2008). Effect of Green and Ripe Carica papaya Epicarp Extract on Wound Healing and during Pregnancy. Food and Chemical Toxicology. 46:2384-2389.
Das, S., Pal, S., Mujib, A. and Dey, S. (1999). Biotechnology of Medicinal Plants- Recent Advances and Potential. Hyderabad, United Kingdom. P. 992.
Devaki, K., Beulah, U., Akila, G. and Gopalakrishnan, V. K. (2012). Effect of Aqueous Extract of Passiflora edulis on Biochemical and Hematological Parameters of Wistar Albino Rats. Toxicology international. 19(1):63–67
Enegide, C., David, A. and Fidelis, S.A. (2013). A New Method for Determining Acute Toxicity in Animal Models. Toxicology International. 20(3):224-226.
Gajalakshmi, S., Vijayalakshmi., S. and Rajeshwari, D.V. (2012). Phytochemical and Pharmacological Properties of Annona muricata: A Review. International Journal of Pharmacy and Pharmaceutical Sciences. 4(2):3-6.
Ingale, A.G. and Hivrale, A.U. (2010). Pharmacological Studies of Passiflora sp. and their bioactive compounds. African Journal of Plant Science. 4(10): 417-426.
Parle, M. and Gurditta, A. (2011). Basketful Benefits of Papaya. International Research Journal of Pharmacy. 2:6-12.
Raj, J., Chandra, M., Dogra, T. D., Pahuja, M., & Raina, A. (2013). Determination of Median Lethal Dose of Combination of Endosulfan and Cypermethrin in Wistar Rat. Toxicology international 20(1): 1-11.
Randhawa, M.A. (2009). Calculation of LD50 Values from the Method of Miller and Tainter, 1944. Journal of Ayub Medical College Abbottabad. 21(3):184-185.
Sandhu, D.S. and Heinrich, M. (2005). The Use of Health Foods, Spices and Other Botanicals in the Sikh Community in London. Phytotherapy Research. 19:633-642.
Valcke, M. and Bouchard, M. (2009). Determination of No-Observed Effect Level (NOEL)-Biomarker Equivalents to Interpret Biomonitoring Data for Organophosphorus Pesticides in Children. Environmental Health. 8(5): 1-12.
Published
2023-05-11
How to Cite
INEGBENOSE GODWIN, I., MARY UGUNNUSH, E., KELVIN OJEBAH, C., & OMO FORTUNATE, R. (2023). STUDY OF LETHAL DOSE (LD50) DETERMINATION AND BODY/ ORGAN WEIGHT OF RATS ADMINISTERED AQUEOUS EXTRACT OF EQUAL WEIGHT OF PAWPAW (Carica papaya) AND SOURSOP (Annona muricata)LEAVE. GPH-International Journal of Biological & Medicine Science, 6(04), 06-13. https://doi.org/10.5281/zenodo.7923212
Issue
Vol 6 No 04 (2023): GPH-International Journal of Biological & Medicine Science
Section
Articles

Copyright (c) 2023 IHIMIRE INEGBENOSE GODWIN

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Author(s) and co-author(s) jointly and severally represent and warrant that the Article is original with the author(s) and does not infringe any copyright or violate any other right of any third parties, and that the Article has not been published elsewhere. Author(s) agree to the terms that the GPH Journal will have the full right to remove the published article on any misconduct found in the published article.