INHIBITOR BIOSENSOR SYSTEMS IN DYNAMIC MODE
Abstract
The biosensor amperometric transducers can work in the case of three basic types of reversible inhibitor enzyme systems – with competitive inhibition, with non-competitive inhibition and mixed inhibition. Tipicaly they work in static mode. Now they are investigated in dynamic mode. In the paper are investigated the influence of starting concentration of inhibitor over output current of the biosensor with three type inhibition enzyme kinetic in dynamic mode. Those kinetic is generally discussed in terms of a simple extension to the Michaelis-Menten reaction scheme. The biosensor is amperometric product sensitive. Solving system of non-linear partial differential equations is reseived in three dimensional size and the concentration profiles of substrate S(x,t), inhibitor I(x,t) and product P(x,t) are reseived. The models are described in non stationary diffusion conditions. The systems of non-linear differential partial equations are solved numerically in MATLAB medium. In the 3D vision are given reagents concentration changing in the active membrane.
Downloads
References
on the Response of Amperometric biosensors. Sensors, 3(7), 248-262.
Baronas R, F.Ivanauskas, J.Kulys. (2003). Computer Simulation of the Response of
Amperometric biosensor in Stirred and non Stirred Solution. Nonlinear Analysis: Modelling and
Control, 8(1), 3-18
Baronas R and J.Kulys, (2006). Modelling of Amperometric Biosensors in the Case of
Substrate Inhibition, Sensors , 1513-1522
Chaplin M. and C. Bucke, (1990). Enzyme Technology, Cambridge University Press, 1990
Lee S. and Won-Yong Lee, . (2002). Determination of Heavy Metal Ions Using Conductometric
Biosensor Based on Sol-Gel-Immobilized Urease, Bull. Korean Chem. Soc. Vol. 23, No. 8
p.1169
Lívia Maria da Costa Silva et al (2013). ). Biosensors for Contaminants Monitoring in Food
and Environment for Human and Environmental Health, chapter of a book,
http://dx.doi.org/10.5772/55617
Author(s) and co-author(s) jointly and severally represent and warrant that the Article is original with the author(s) and does not infringe any copyright or violate any other right of any third parties, and that the Article has not been published elsewhere. Author(s) agree to the terms that the GPH Journal will have the full right to remove the published article on any misconduct found in the published article.