Determination of ProthrombinGene G20210A Mutation in Deep Venous Thrombosis among Sudanese Patients - Khartoum State
Abstract
Background: Deep vein thrombosis (DVT) is serious disorders contributing to increased morbidity and mortality worldwide. However, there is, little genetic data from Africa including Sudan. So this study was conducted to investigate the relationship between genetic mutations G20210A in the prothrombin coagulation factor and deep venous thrombosis in Sudanese patients.
Methods: This is a cross-sectional study design wascarried out in Khartoum state in the period from December 2014 to May 2018. One hundred of patient group and fifty healthy one were enrolled inthis study. Mutation was detected using conventional PCR depending on restriction enzyme polymorphism technique.
Results: The mutant allele (G/G) was found in 4 (4%) of patients nevertheless, there is no mutant allele was present in control group (P.value=0.152). Moreover, the mixed allele (G/A) was not detected in patients and healthy control.Exclusively, the control group showed only the wild type (A/A).
Conclusion: This study noted a few mutant alleles in patients group and only the wild type (A/A) in control one. However, carrying out a genome-wide associated study is recommended to determine relationship betweenprothrombin gene mutation and frequency of deep vein thrombosisin Sudanese population.
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