• I Made Sudarma Lecturer staff at the Agroecotechnology Study Programe, Faculty of Agriculture
  • Ni Wayan Suniti Staff Lecturer in Agroecotechnology Study Program Faculty of Agriculture
  • dan Ni Nengah Darmiati Staff Lecturer in Agroecotechnology Study Program Faculty of Agriculture
Keywords: Neoscytalidium dimidiatum, exophyte, endophyte, diversity index, dominance Index


Pineapple rot disease is one of the most significant postharvest diseases caused by Neoscytalidium dimidiatum (Penz.) Crous & Slippers, which can lead to substantial crop losses. There is a complex of microbes that may influence the growth and development of pineapple rot disease under natural conditions. This research was conducted to determine the role of exophytic and endophytic microbes in preventing pineapple rot. In healthy fruit, 45 isolates of exophytic fungi and 24 isolates of endophytic fungi were isolated from exophytes and endophytes, respectively. The dominant fruit exophytes of Thermasporomyces composti (Actinomycetes) were 15 isolates, while the dominant healthy fruit endophytes were as many as 12 isolates of Colletotrichum sp. Exophytes and endophytes were also found on healthy leaves, where the dominant exophytes were 45 isolates and the dominant endophytes were 24 isolates. Rhizopus sp. 36 and Rhizopus sp. 12 are both exophytes and endophytes. The diversity and dominance indices of fruit exophytic fungi were 1,767 and 0.800, respectively, while the diversity and dominance indices of fruit endophytic fungi were 1,386 and 0.6875, respectively. The diversity index for healthy leaf exophytes was 0,7201, with a dominance index of 0.3467, while the diversity index for endophytic microbes was 1.386, with a dominance index of 0.688. The inhibition of pathogens by fruit exophytic microbes ranged from 66.67 to 88.89 percent, with A. flavus1 achieving the highest level of 88.89 percent. Rhizopus sp. and Neurospora sp., both found in healthy leaf habitats, inhibit the growth of pathogens in vitro by 88.89%. A. flavus exhibited the most effective in vivo inhibition, with an  percentage of inhibition of 3.8+1.39%.


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Author Biographies

I Made Sudarma, Lecturer staff at the Agroecotechnology Study Programe, Faculty of Agriculture

Udayana University, Jl. PB. Sudirman Denpasar, Bali, Indonesia

Ni Wayan Suniti, Staff Lecturer in Agroecotechnology Study Program Faculty of Agriculture

Udayana University Jl. PB. Sudirman Denpasar-Bali

dan Ni Nengah Darmiati, Staff Lecturer in Agroecotechnology Study Program Faculty of Agriculture

Udayana University Jl. PB. Sudirman Denpasar-Bali


Barnett, H.L. and B.B. Hunter. 1998. Illustrated Genera of Imperfect Fungi. APS Press. The American Phytopathological Sociey. St Paul, Minnesota.

Dolar, F.S. 2001. Antagonistic effect of Aspergillus melleus Yukawa on soilborne pathogens of Chickpea. Tarim Bilimleri Dergisi, 8(2): 167-170.

Indrawati. G., R.A. Samson, K. Van den Tweel-Vermeulen, A. Oetari dan I. Santoso. 1999. Pengenalan Kapang Tropik Umum. Yayasan Obor Indonesia. Universitas Indonesia (University of Indonsia Culture Collection) Depok, Indonsia dan Centraalbureau voor Schirmmelcultures, Baarn, The Netherlands.

Joy P.P. and G. Sindhu. 2012. Pineapple Research Station (Karala Agricultural University) Vazhakulam-686 670, Mavattupuzha, Emakulam, India.
Kuruppu, M. Y. Siddiqui, L. L. Kong, K. Ahmed, A. Ali. 2020. First report of postharvest stem end rot disease on MD2 pineapple fruits caused by Neoscytalidium dimidiatum in Malaysia.Laboratory of Sustainable Agronomy and Crop Protection, Institute of Plantation Studies, Universiti Putra Malaysia, Serdang 43400 Selangor, Malaysia.
Langvad, F. 1980. A simple and rapid method for qualitative and quantitative study of the fungal flora of leave. Canadian Journal of Botany 26: 666-670.
Miyadoh, S. 1997. Atlas of Actinomycetes. Asakura Publishing Co Ltd. Japan.
Mojica-Marin, V., H. A. Luna-Olvera, C. Fco, Sandoval-Coronado, B. Pereyra- Alférez, H. Lilia, Morales-Ramos, E. Carlos, Hernández-Luna and G. O. Alvarado-Gomez. 2008. Antagonistic activity of selected strains of Bacillus thuringiensis against Rhizoctonia solani of chili pepper. African Journal of Biotechnology, 7 (9): 1271-1276.
Odum, E.P. 1971. Fundamentals of Ecology. Third Edition. W.B. Saunders Company.Philadelphia, Toronto, London. Toppan Company, Ltd. Tokyo, Japan.
Pirzan, A.M., dan P. R. Pong-Masak. 2008. Hubungan Keragaman Fitoplankton dengan Kualitas Air di Pulau Bauluang, Kabupaten Takalar, Sulawesi Selatan. Biodiversitas, 9 (3) 217-221 (Indonesia language).
Pitt, J.I. and A.D. Hocking. 1997. Fungi and Food Spoilage. Blackie Avademic and Professional. Second Edition. London-Weinhein-New York-Tokyo-Melboune-Madras.
Rad, J.E., M. Manthey and A. Mataji. 2009. Comparison of Plant Species Diversity with Different Plant Communities in Deciduous Forests. Int. J. Environ. Sci. Tech, 6(3): 389-394.
Rohrbach, K. G. and D.J. Phillips. 1990. Postharvest Diseases of Pineapple. Acta Horticulturae 269: 503-508.
Samson, R.A., E.S. Hoekstra, and C. A.N. Van Oorschot. 1981. Introduction to Food-Borne Fungi. Centraalbureau Voor-Schimmelcultures. Institute of The Royal Netherlands. Academic of Arts and Sciences.

Sudarma I M., N.W. Suniti dan N.N. Darmiati. 2018. Exophytic and endophytic fungus that potential as biocontrol agents on Lasiodiplodia theobromae caused fruit rot at sugar-apple. Int.J.Curr.Microbial.App.Sci.8(2): 131-142.
Sudarma, I M., N. N. Darmiati and N.W. Suniti. 2019. Fungus and Actinomycetes Diversity of Exophytic and Endophytic in Red Grape and its Inhibition Ability to Pathogen Aspergillus niger (Caused Rot Fruit Grape). Int.J.Curr.Microbiol.App.Sci 8(10): 2442-2451.

Sudarma, I M., N. W. Suniti and N. N. Darmiati. 2020. Use of exophytic microbial on the controlof fruit rot diseases of mango (Lasiodiplodia theobromae). Int.J.Curr.Microbiol.App.Sci (2020) 9(4): 845-854.
Sudarma, I M., N.W. Suniti and N.N. Darmiati. 2021a. Molecular identification of Anthracnose pathogen (Colletotrichum musae) in banana fruits and the use exophytic and endophytic fungi to control the pathogen. GPH Journal of Agriculture and Research. 04(11): 01-11.
Sudarma, I M., N.N. Darmiati, and N. W. Sunii, 2021b. Control of papaya rot disease by using exophytic and endophytic fungi which is environmentally friendly. Int. J. Curr. Microbial. App. Sci 10(09): 600-612.
Sudarma I M., N.W. Suniti and N.N. Darmiati. 2021c. The potential of exophyitic microbials in control of black rot disease of mangoes (Aspergillus niger). GPH – Journal of Agiculture and Research 04(01): 10-18.
Tauruslina,E,Trizelia ,Yaherwandi dan Hasmiandy ,H. 2015. Analisis keanekaragaman hayati musuh alami pada eksosistem pada sawah di daerah endemik dan non - endemik Wereng Batang Cokelat Nilaparvata lugens di Sumatera Barat. Pros Sem Nas Masy Biodiv Indon 1(3): 581 – 589 (Indonesian language).
How to Cite
Sudarma, I. M., Wayan Suniti, N., & Nengah Darmiati, dan N. (2022). THE ROLE OF EXOPHYTIC AND ENDOPHYTIC MICROBES IN CONTROLLING FRUIT ROT DISEASE ON PINEAPPLE (Ananas comosus (L.) Merr. GPH-International Journal of Agriculture and Research E-ISSN 2805-4362 P-ISSN 2805-4340, 5(07), 07-18.