MOLECULAR IDENTIFICATION OF ANTHRACHNOSE PATHOGEN (Colletotrichum musae) IN BANANA FRUITS AND THE USE OF EXOPHITIC AND ENDOPHYTICFUNGI TO CONTROL THE PATHOGEN
Banana rot disease is often found in Bali, especially bananas are the main means for completing religious ceremonies in Bali. Banana rot often spoils during storage or post-harvest. The results showed that the cause of banana rot disease was Colletrotrichummusae with DNA fragments measuring 650 bp successfully amplified using universal primers ITS1/ITS4. The highest in vitro inhibition of exophytic fungi against pathogens was achieved by A. niger, Neurospora sp. and Rhizopus sp. each with an inhibitory power of 100%, the lowest was achieved by Neurospora sp. at the time of days after inoculation (DAI) by 50%. While the inhibition of endophytic fungi against pathogens was Rhizopus sp. with inhibition of 100% from 1 DAI to 4 DAI followed by A. niger at 100% at 3 and 4 DAI. The results of the in vivo inhibition of selected exophytic and endophytic fungi against pathogens were as follows: the highest inhibitory power was C (Rhizopus sp.1) of 94±5.48%, followed by treatment A (A. niger 1) of 92±8.37%, treatment B (A. niger 2) was 64±5.48% %, treatment E (Rhizopus sp. 2) was 54±5.48%, and the least inhibition was treatment D (Neurospora sp. 1) by 44±5.48%.
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