THE POTENTIAL OF EXOPHYTIC MICROBIALS IN CONTROL OF BLACK ROT DISEASE OF MANGOES (Aspergillus niger)

  • I Made Sudarma Lecturer
  • Ni Wayan Suniti Lecturer
  • Ni Nengah Darmiati Lecturer
Keywords: antagonist, black rot, exophytic, inhibition, postharvest

Abstract

Postharvest black rot disease of mangoes is the most important disease in Bali, especially fruit that has been used for religious ceremonies cannot last long if stored, therefore there is a desire to research efforts to prevent fruit rot with antaginist microbes. Postharvest fruit rot disease in mangoes is Aspergillus niger. In our study, we looked for the exophytic fungi and tested their inhibition of pathogen with exophytic microbes both in vitro and in vivo. The results showed that exophytic microbes found in healthy mangoes include: Rhizopus sp. the number of colonies was 24 x 102 cfu, while Nucordia sp., A. flavus, and A. niger with 18 x 102cfu colonies, and Streptomyces sp. with a colony of 12 x 102cfu. The highest microbial inhibition test results of exophyte against A. niger were obtained from Rhizopus sp. 1 and Rhizopus sp. 4 at 3 and 7 after inoculation.  The results of the in vivo antagonistic inhibition test against pathogens (A. niger) were the highest C treatment (Rhizopus sp. 2) and very significantly different from K + P treatment (control with pathogens).

Downloads

Download data is not yet available.

Author Biographies

I Made Sudarma, Lecturer

Lecturer staff at the Agroecotechnology Study Programe, Faculty of Agriculture, Udayana University, Jl. PB. Sudirman Denpasar, Bali, Indonesia

Ni Wayan Suniti, Lecturer

staff at the Agroecotechnology Study Programe, Faculty of Agriculture, Udayana University, Jl. PB. Sudirman Denpasar, Bali, Indonesia

Ni Nengah Darmiati, Lecturer

staff at the Agroecotechnology Study Programe, Faculty of Agriculture, Udayana University, Jl. PB. Sudirman Denpasar, Bali, Indonesia

References

Barnett, H.L. and B.B. Hunter. 1998. Illustrated Genera of Imperfect Fungi. APS Press. The American PhytopathologicalSociey. St Paul, Minnesota.
Dolar, F.S. 2001. Antagonistic effect of Aspergillus melleus Yukawa on soilborne pathogens of Chickpea. TarimBilimleriDergisi, 8(2): 167-170.
Endrawati, D., dan E. Kusumaningtyas. 2017. BeberapaFungsi Rhizopus spdalamMeningkatkan Nilai NutrisiBahanPakan.Wartazoa 27(2): 081-088 (Indonesian language)
Haggag W. M. 2010. Mango Diseases in Egypt. Agriculture and Biology Journal of North America 1(3): 285-289.
Indrawati. G., R.A. Samson, K. Van den Tweel-Vermeulen, A. Oetari dan I. Santoso. 1999. PengenalanKapangTropikUmum. Yayasan Obor Indonesia. Universitas Indonesia (University of Indonsia Culture Collection) Depok, Indonsia dan CentraalbureauvoorSchirmmelcultures, Baarn, The Netherlands (Indonesian language)
Miyadoh, S.N., Tsuchizaki., J. Ishikawa and Hotta. 2002. Digital Atlas of Actinomycetes. The Society for Actinomycetes. Japan.
Mojica-Marin, V., H. A. Luna-Olvera, C. Fco, Sandoval-Coronado, B. Pereyra- Alférez, H. Lilia, Morales-Ramos, E. Carlos, Hernández-Luna and G. O. Alvarado-Gomez. 2008. Antagonistic activity of selected strains of Bacillus thuringiensis against Rhizoctonia solani of chili pepper. African Journal of Biotechnology, 7 (9): 1271-1276.
Pitt, J.I. and A.D. Hocking. 1997. Fungi and Food Spoilage. Blackie Avademic and Professional. Second Edition. London-Weinhein-New York-Tokyo-Melboune-Madras.
Prakash, O.M., A.K. Misraand and P.K. Shukla. 2011. Post-harvest diseases of mango and their management. Global Confrence on Augmenting Production and Utulization of Mango: Biotic and Abiotic Stress. P: 137-144.
Samson, R.A., E.S. Hoekstra, and C. A.N. Van Oorschot. 1981. Introduction to Food-Borne Fungi. CentraalbureauVoor-Schimmelcultures. Institute of The Royal Netherlands. Academic of Arts and Sciences.
Sharma, R. 2012. Pathogenecity of Aspergillus Niger in Plants. Cibtech Journal of Microbiology 1(1): 2319-3867.
Stone E.K., J.D. Polishook and J.F. White. 2014. Endophytic Fungi. The State University of New Jersey. 241-270.
Sudarma I M., N. N. Darmiati and N. W. Suniti. 2019. Fungus and Actinomycetes Diversity of Exophytic and Endophytic in Red Grape and its Inhibition Ability to Pathogen Aspergillus niger (Caused Rot Fruit Grape). Int. J. Curr. Microbiol. App. Sci 8(10): 2442-2451.
Sudarma I M., N. W. Suniti, N.N. Darmiati and I G.N. Bagus. 2018. The Diversity of Exophytic Fungus in Sugar-Apple Plant and their Inhibition Ability on Lasiodiplodiatheobromae(Cause of Srikaya Fruit Rot) In Vitro. Int. J. Curr. Res. Biosci. Plant Biol. 5(10), 32-38.
Wulandari, D., L. Sulistyawati, dan A. Muhibuddin. 2014. KeanekaragamanJamurEndofit pada TanamanTomat (Lycopersicum Esculentum Mill.) dan KemampuanAntagonisnyaTerhadapPhytophthora Infestans, Jurnal HPT 2(1): 110-118(Indonesian language).
Wulandari, D.E., Asrul, dan I. Lakani. 2016. SeleksiJamurAntagonisAspergillus Niger Dari BeberapaLahan Perkebunan KakaoUntukMengendalikanPhytophthora Palmivora. J. Agroland23 (3): 233 – 242 (Indonesian language).
Published
2021-01-17
How to Cite
Sudarma, I. M., Suniti, N. W., & Darmiati, N. N. (2021). THE POTENTIAL OF EXOPHYTIC MICROBIALS IN CONTROL OF BLACK ROT DISEASE OF MANGOES (Aspergillus niger). GPH-International Journal of Agriculture and Research E-ISSN 2805-4362 P-ISSN 2805-4340, 4(01), 10-18. Retrieved from http://gphjournal.org/index.php/ar/article/view/352