The Efficacy of Rhabditis blumi nematode on larvae Sesamia cretica Led. under laboratory conditions.

  • عثمان حسين علي الرفيعي
  • عبد الباقي السيد علي
  • سيف الدين محمد خير
  • محمد زيدان خلف
Keywords: Corn Stalk Digger, Sesamia cretica, Nematoda Insect Nurse, Rhbditis blumi, Bio-Control

Abstract

Laboratory experiments were carried out on the effect of Rhbditis blumi in the larvae of the sesamia cretica corn stem digger In the experiments, three concentrations of nematodes 50, 100, 150 infectious phases/ml were used and treated in comparison to water only, the experiments were carried out in two ways, one treated the larvae directly and the other treated the larvae's food and calculated the number of dead larvae daily after treatment The results indicated that the nematodo a solution containing 150 infectious stages/ml led to the highest kill rate of 68%, 88% when using nematodes in the methods of food treatment and spraying larvae directly, respectively, the results showed The increase in the number of infectious phases in the spray solution led to an increase in the rate of larvae kill, the concentration gave 50 infectious phases the lowest kill rate of 16% and 32% in the treatment of larvae food and direct spraying on larvae respectively compared to the concentration of 150 infectious phases / ml, which led to the highest kill rate in the larvae of the old corn leg digger The killing rate has gradually increased over time after the treatment, reaching the highest Level after 5 days of transaction

These results can be used in the field of S. cretica corn stem excavator control using Nematoda R. blumi as part of the integrated control programmes for this instalment.

Author Biographies

عثمان حسين علي الرفيعي

College of Agricultural Studies, Sudan University of Science and Technology, Khartoum, Sudan

عبد الباقي السيد علي

College of Agricultural Studies, Sudan University of Science and Technology, Khartoum, Sudan

سيف الدين محمد خير

College of Agricultural Studies, Sudan University of Science and Technology, Khartoum, Sudan

محمد زيدان خلف

Agricultural Research Department, Ministry of Science and Technology, Baghdad, Republic of Iraq

References

المصادر:
المجموعة الاحصائية السنوية. 2006. الجهاز المركزي للاحصاء. جمهورية العراق.
العامري، ناصر معروف ناصر , 2011. تأثير المكافحة الكيميائيةٌ لحفار ساق الذرة Sesamia cretica Led. في نمو وانتاجية محصول الذرة الصفراء Zea mays L. في محافظة القادسية. مجلة الفرات للعلوم الزراعية. 130 – 123: (1) 3 - .
مؤنس، عبد المحسن حسين. 1978. دراسات حقليه عن الكثافة العددية لحفار ساق الذرة في وسط العراق ومقاومتها بالطرق الزراعية. رسالة ماجستير، كلية الزراعة جامعة بغداد.
الراوي، خاشع محمود وخلف الله، عبد العزيز محمد (1980). تصميم وتحليل التجارب الزراعية. دار الكتب للطباعة والنشر، جامعة الموصل، 488 صفحة.
الجبوري إبراهيم جدوع، وصبا جعفر صالح .2001. أول تسجيل لنيماتودا طفيلية على حفار ساق النخيل ذو القرون الطويلة وحفار عذق النخيل في العراق. مجلة البصرة لابحاث نخلة التمر، المجلد 1: العدد 1.
FAO. 2006. FAOSTAT, statistical database.http://www.FAO.org.
Hwan kim, Hyeong, Sung Hun Youn, Taek Su Shin, 2012. Biological control potentials of insect-parasitic nematode Rhabditis blumi (Nematoda: Rhabditida) for major cruciferous vegetable insect pests. Applied Entomology and Zoology. 47 , 389 – 397 , Cite this article .
Jakubowska, A., Van Oers, M.M., Ziemnicka, J., Lipa, J.J., Vlak, J.M. (2005). Molecular characterization of Agrotis segetum nucleopolyhedrovirus from Poland. J. Invertebr. Pathol., 90,64–68.
Khalaf. Mohammed Z. Ahmad M. Tareq Falah H. Naher , Adnan H. Salman and Haitham S. Khalaf.(2018) BIOLOGICAL CONTROL OF THE DATE PALM TREE BORERS, ORYCTES AGAMEMNONARABICUS (COLEOPTERA: SCARABAIDAE: DYNASTINAE), Journal homepage: www.pakentomol.com
Masaaod Zolfagharian , Asaeedizadeh and Habib Abbasipour . 2016. Efficacy of Two Entomopathogenic Nematode Species as Potential Biocontrol Agents against the Diamondback Moth, Plutella xylostella (L.) DOI:10.18641/jbc/30/2/88697Corpus ID: 88861389
Nithiskarani , Anita , and Vetrivelkalai , Selena Nelson . 2019 . Biocontrol potential of entomopathogenic Nematodes, Steinernema glaseri (Steiner, 1929) and Heterorhabditis indica (Poinar, Karunakar & David, 1992) against brinjal ash weevil (Myllocerus subfasciatus) . Published 2019. Biology.
Osman, Assem , Alreedy , EL-Ghareeb , Basry ,Rastogia and Kalaji . 2015 . Development of insect resistant maize plants expressing a chitinase gene from the cotton leaf worm, Spodoptera littoralis. 2015 Dec 14; 5:18067. doi: 10.1038/srep18067.
Ricano, J., B. Guerri-Agullo, M. J. Serna-Sarrias, G. Rubio-Llorca, L. Asensio, P. Barranco and V. Lopez-llorcal,2013. Evaluation of the pathogenicity of multipleisolates of Beauveria bassiana (Hypocreales:Clavicipitaceae) on Rhynchophorus ferrugineus(Coleoptera: Dryophthoridae) for the assessment of asolid formulation under simulated field conditions. -Fla. Entomol. 96: 1311-1324.
SAS Institute Inc. SAS state guide for personal computer. Version 6 ed. SAS Institute, Cary, NC. USA. (2010). Abbott, W. S .1925. Method for computing the effectiveness of an insecticide. J. Econ. Enstool. 18: 265 – 267.
White, P. J. and L. A. Johnson. 2003. Corn: Chemistry and Technology.2nd Edn., American Association of Cereal Chemists, St. Paul, MN., USA., ISBN-13: 9781891127335, PP: 892.
Yun, J.S., Kim, H.H., Kim, D.W., Lee, S.M., Kim, D.S., Lee, D.W. (2004). Pathogenicities of entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae against lepidopterous insect pests, Agrotis segetum, Artogeia rapae, Mamestra brassicae, Plutella xylostella,Spodoptera exigua and Spodoptera litura. Asian J. Turfgr. Sci., 18, 221–229.
Published
2020-10-31
How to Cite
علي الرفيعيع. ح., السيد عليع. ا., محمد خيرس. ا., & خلفم. ز. (2020). The Efficacy of Rhabditis blumi nematode on larvae Sesamia cretica Led. under laboratory conditions. GPH - IJAR International Journal of Agriculture and Research, 3(10), 12-23. Retrieved from http://gphjournal.org/index.php/ar/article/view/319